![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1824008:Design
Designed by: Wenbo Xu Group: iGEM15_XJTLU-CHINA (2015-09-06)
Consensus Promoter T7 + RNA Thermometer U6
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 155
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal SpeI site found at 155 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal BamHI site found at 141 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 155
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 155
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequence
Source
T7 promoter and U6 were from registry.